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CONFOBRIGHT

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Improving the performance of confocal microscopy

profilePhoto   Christine Melay
Linksium Contact Christine Melay +33 (0)6 21 77 19 90 christine.melay@linksium.fr
Figures site web 25fev20

Benefits

  • Improved performance of quantitative confocal microscopy
  • Use of a deformable mirror
  • Wavefront corrector

Key words

  • Adaptive optics
  • Confocal microscopy
  • Fluorescence correlation
  • Spectroscopy

Intellectual Property

  • 1 patent
  • 1 software
  • 1 knowhow

Laboratory

  • LIPHY

Institutions

  • CNRS
  • UGA

Linksium Continuum

  • Maturation
  • Commercialization

Results

  • Available licenses

Context

Basic biomedical research requires precise measurements of concentration and molecular dynamics in optically complex media: cells, 3D tissues... Confocal microscopy is then widely used, but lacks internal reference and means of correcting aberrations.

Technology

We have developed a technique for correcting the light wavefront. Based on the optimization of molecular brightness and using a deformable mirror, it will allow the commercial confocal microscope to adapt to each biological sample and produce reliable images and quantitative measurements.

Advantages

  • Confocal 3D confocal microscopy will thus become quantitative in-situ
  • Measurement results will be reproducible and comparable between laboratories
  • Existing microscopes can be improved by adding an accessory
  • The resolution and contrast of tissue images will be improved

State of progress

The functional prototype is already built in the research laboratory and the technology is validated by specific applications. The technology transfer enters its final phase with the construction of a pre-industrial prototype based on a commercial confocal microscope.

Applications

The innovation will enable deeper, more resolving and sensitive confocal imaging in optically heterogeneous tissues and specimen holders. It will thus allow measurements of concentrations and molecular mobility using fluorescence correlation spectroscopy methods.