Transparent mouse liver sample, GFP labelled, 40 µm deep. By optimizing the shape of the deformable mirror so that the chosen metric is maximum, the intensity, contrast and resolution of the image are improved (left, without adaptive optics, right, with)
Basic biomedical research requires precise measurements of concentration and molecular dynamics in optically complex media: cells, 3D tissues, … Confocal microscopy is then widely used but lacks internal reference and means of correcting aberrations.
We have developed a technique for correcting the light wavefront. Based on the optimization of molecular brightness and using a deformable mirror, it will allow the commercial confocal microscope to adapt to each biological sample and produce reliable images and quantitative measurements.
Confocal 3D confocal microscopy will thus become quantitative in-situ; measurement results will be reproducible and comparable between laboratories; existing microscopes can be improved by adding an accessory; the resolution and contrast of tissue images will be improved.
The functional prototype is already built in the research laboratory and the technology is validated by specific applications. The technology transfer enters its final phase with the construction of a pre-industrial prototype based on a commercial confocal microscope.
The innovation will enable deeper, more resolving and sensitive confocal imaging in optically heterogeneous tissues and specimen holders. It will thus allow measurements of concentrations and molecular mobility using fluorescence correlation spectroscopy methods.