Cell health monitoring with a synthetic biosensor
- Monitoring of cellular metabolism
- Screening for toxicity and efficacy of compounds
- Sensitive, real-time
- Multiplexing with other fluorescent labels
- Genetically-encoded fluorescent reporter
- Single cell biology
- Spatiotemporal readout
- Cell-based screening assays
- 1 patent
Energy is a crucial requirement for life, and energy state is a good indicator for cellular health. However, methods that quantify cellular energy state and its response to specific compounds or treatments lack sensitivity and the possibility of live cell monitoring at spatiotemporal resolution.
AMPfret technology exploits the endogenous cellular mechanism for detection of energy stress, based on the protein AMPK. The genetically-encoded AMPfret sensor translates deviations from physiological energy levels into a fluorescence signal (FRET) for reversible, continuous on-line monitoring of energy state in single cells and cell populations.
AMPfret technology extends analytics beyond classical cell viability tests. It detects cellular dysfunctions already at a premature, not yet detrimental state, including transient or low-level adverse effects. Such fine-tuned analysis of cellular health is a powerful tool to evaluate toxicity or therapeutic efficiency of individual compounds or more complex mixtures.
State of progress
The patented and published proof-of-concept (Pelosse et al., Nature Communications 2019) demonstrates the efficiency of AMPfret in different cell lines.
The HealthProbe Project is developing a set of standardized tests on both human cell lines and primary cells with stable or transient AMPfret expression.
The applications cover the whole range of cytotoxicity and efficacy tests of compounds to preclinical drug screening in cosmetic and pharmaceutical industry.
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