Easy-to-use quantitative FRET analysis
- Quantitative FRET measurements
- Available for a standard fluorescence microscope
- One-step calibration directly on the sample
- Robust pixelwise analysis
- Non-specialists users
- Cell analysis
- Stoichiometry information available
- 1 software
- Available licenses
In biology, the main interest of FRET (Fluorescence Resonance Energy Transfer) is to measure a specific biochemical activity directly on living cells. However, the massive use of FRET biosensors is hampered by the difficulty in setting up the experiments and, above all, by a complex analysis.
Sensofluo is a software solution that proposes a new method of calibration of FRET (fluorophore + instrument) without the need to normalise before/after (relative measurement) and allowing direct comparison of different experimental conditions.
This calibration allows quantitative measurements.
Sensofluo solves the complexity of FRET analysis and allows non-specialists to access this technology with a reliable quantitative approach. The result is an absolute FRET value, independent of the experimental setup, only dependent on the biology.
State of progress
Sensofluo is a plug and play software that can be added to any type of FRET data analyser and is compatible with all types of microscopes. The POC was illustrated in a publication : A Coullomb et al. "QuanTI-FRET: a framework for quantitative FRET measurements in living cells" Scientific Reports (2020).